Rat HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit
The Rat HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit is a sophisticated assay meticulously crafted for the quantitative assessment of Heat Shock Protein 70 levels in diverse rat biological samples. Heat Shock Protein 70, also known as HSPA9, is a critical chaperone protein involved in protein folding, cellular stress responses, and protection against various physiological insults. HSP-70 plays a pivotal role in maintaining protein homeostasis and promoting cellular survival under stressful conditions by assisting in proper protein folding and preventing aggregation. This highly specialized ELISA kit empowers researchers to accurately measure HSP-70 levels, shedding light on its functions in cellular homeostasis, stress adaptation, and disease development.
By quantifying the expression of HSP-70, researchers can unravel the protein's intricate roles in cellular health and disease progression, resulting in valuable insights that can guide novel therapeutic strategies and interventions. Known for its unparalleled sensitivity and specificity, the Rat HSP-70/HSPA9 ELISA Kit ensures the generation of precise, reproducible, and trustworthy data, fostering the advancement of scientific research. With its robust performance and user-friendly features, this kit represents an excellent tool for researchers investigating the involvement of HSP-70 in cellular stress responses, proteostasis, and disease pathologies.
Product Name:
Rat HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit
SKU:
AEES00425
Target:
Rat HSP-70/HSPA9 (Heat Shock Protein 70)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-70/HSPA9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-70/HSPA9. You can calculate the concentration of Rat HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
88-101
86-100
Average (%)
104
93
92
1:4
Range (%)
98-115
83-93
94-107
Average (%)
106
88
99
1:8
Range (%)
93-106
83-94
95-108
Average (%)
100
89
101
1:16
Range (%)
98-109
83-96
92-105
Average (%)
104
89
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.4
9.5
1.0
2.5
9.5
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
4.17
4.21
10.0
4.0
4.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat HSP-70/HSPA9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Rat HSP-70/HSPA9 and analogues was observed.
