Rat hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit
The Rat hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit is meticulously designed to enable the precise and quantitative measurement of high-sensitivity CRP levels, a key biomarker of systemic inflammation and a crucial indicator of various pathological conditions, in diverse biological samples obtained from rat subjects.
High-sensitivity CRP serves as an important inflammatory marker indicative of inflammation, infection, and associated adverse health conditions. Accurate quantitation of hs-CRP levels through this specialized ELISA kit plays a critical role in assessing and monitoring inflammatory processes within the body. High levels of hs-CRP are linked to cardiovascular diseases, autoimmune disorders, infections, and several other health complications. Hence, the quantitative data obtained from this ELISA assay can provide valuable insights into the inflammatory status, disease progression, and overall health condition in rat models. The Rat hs-CRP ELISA Kit by Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate, reproducible, and reliable results. Manufactured under strict quality control measures, this kit assures robust performance and simple usability, making it an excellent tool for researchers exploring the role of high-sensitivity CRP in inflammatory responses and associated health disorders.
Product Name:
Rat hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit
SKU:
AEES00518
Target:
Rat hs-CRP (high-sensitivity C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat hs-CRP. You can calculate the concentration of Rat hs-CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-102
86-94
93-105
Average (%)
96
90
97
1:4
Range (%)
101-113
96-105
92-106
Average (%)
105
101
98
1:8
Range (%)
96-108
99-109
92-106
Average (%)
104
102
99
1:16
Range (%)
95-105
100-110
100-108
Average (%)
99
104
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-97
93
EDTA plasma (n=5)
97-109
105
Cell culture media (n=5)
90-101
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.13
56.75
235.5
20.39
60.86
222.49
Standard deviation
1.2
2.81
12.29
1.07
2.87
9.52
C V (%)
5.96
4.95
5.22
5.25
4.72
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat hs-CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat hs-CRP in samples. No significant cross-reactivity or interference between Rat hs-CRP and analogues was observed.
