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Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit (AEES00435)

SKU:
AEES00435
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
Frequently bought together:

Description

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Product Name:Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit
SKU:AEES00435
Target:Rat HMGB-1 (High Mobility Group Protein B1)
Size:96T
Assay type:Sandwich-ELISA
Assay time:3.5h
Sensitivity:18.75 pg/mL
Detection range:31.25-2000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 12 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 12 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 12 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HMGB-1. You can calculate the concentration of Rat HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)90-10788-9985-97
Average (%)989391
1:4Range (%)92-10485-9884-98
Average (%)989291
1:8Range (%)92-10585-9883-96
Average (%)979088
1:16Range (%)88-9985-9985-97
Average (%)949191
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)87-10394
EDTA plasma (n=5)93-10698
Cell culture media (n=5)91-10497
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)92.38254.88974.9884.22250.98977.51
Standard deviation5.3510.8132.475.5110.3930.01
C V (%)5.794.243.336.544.143.07
Sample type &Sample volume:serum, plasma and other biological fluids; 100μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:This kit recognizes Rat HMGB-1 in samples. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed.

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