Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit
The Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit is a specialized assay designed for the precise quantitative detection of HMGB-1 levels in diverse biological samples. High Mobility Group Protein B1 is a vital nuclear protein that plays a multifaceted role as a DNA chaperone, modulator of transcriptional activity, and mediator of inflammation and immunity. It is often released into extracellular spaces during cellular stress, infection, and inflammatory processes, where it acts as a damage-associated molecular pattern (DAMP) to regulate immune responses.
Accurate measurement of HMGB-1 levels is crucial for unraveling its involvement in diverse physiological and pathological conditions, ranging from inflammation to cancer. The Rat HMGB-1 ELISA Kit provides exceptional sensitivity and specificity, enabling researchers to obtain reliable and reproducible quantitative data. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an indispensable tool for investigating the intricate roles of HMGB-1 in health and disease.
Product Name:
Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit
SKU:
AEES00435
Target:
Rat HMGB-1 (High Mobility Group Protein B1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HMGB-1. You can calculate the concentration of Rat HMGB-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-107
88-99
85-97
Average (%)
98
93
91
1:4
Range (%)
92-104
85-98
84-98
Average (%)
98
92
91
1:8
Range (%)
92-105
85-98
83-96
Average (%)
97
90
88
1:16
Range (%)
88-99
85-99
85-97
Average (%)
94
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.38
254.88
974.98
84.22
250.98
977.51
Standard deviation
5.35
10.81
32.47
5.51
10.39
30.01
C V (%)
5.79
4.24
3.33
6.54
4.14
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HMGB-1 in samples. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed.
