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Rat Histamine Receptor H4 (HRH4) ELISA Kit

SKU:
RTDL00499
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91ZY1
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GPCR105,GPRv53,H4R,HH4R,H4,AXOR35,BG26,G-protein coupled receptor 105
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Histamine Receptor H4 (HRH4) ELISA Kit

The Rat Histamine Receptor H4 (HRH4) ELISA Kit is a valuable tool for researchers looking to accurately measure HRH4 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.Histamine receptor H4 is a key player in mediating histamine's effects on various physiological processes, including immune response regulation and inflammation.

Dysregulation of HRH4 has been linked to inflammatory disorders, allergies, and autoimmune diseases, making it an important target for therapeutic interventions and biomarker studies.By utilizing the Rat Histamine Receptor H4 ELISA Kit, researchers can gain valuable insights into the role of HRH4 in disease pathology and explore potential therapeutic strategies for targeting this receptor.

Product Name:Rat Histamine Receptor H4 (HRH4) ELISA Kit
Product Code:RTDL00499
Size:96 Assays
Target:HRH4
Synonyms:GPCR105, GPRv53, H4R, HH4R, H4, AXOR35, BG26, G-protein coupled receptor 105
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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