Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit (RTES00302)
- SKU:
- RTES00302
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 1.88pg/mL
- Range:
- 3.13-200pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
- Reactivity:
- Rat
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit |
SKU: | RTES00302 |
Target: | Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 1.88 pg/mL |
Detection range: | 3.13-200 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Tissue homogenates,cell lysates and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids. |
Specificity: | This kit recognizes Rat HIF-1α in samples. No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed. |