The Rat Hepc (Hepcidin) ELISA Kit is expertly designed to accurately quantify Hepcidin levels in diverse rat biological samples. Hepcidin is a vital peptide hormone that plays a crucial role in iron regulation within the body. It is primarily synthesized in the liver and acts as a key regulator of iron metabolism, controlling iron absorption in the intestine, iron release from macrophages, and iron entry into circulation.
By measuring Hepcidin levels, researchers can gain valuable insights into iron homeostasis, erythropoiesis, and the pathophysiology of iron-related disorders. Accurate quantification of Hepcidin is essential for unraveling its intricate role in iron balance and its implications in various health conditions. The Rat Hepc ELISA Kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results critical for advancing iron metabolism research. Manufactifiable to stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent tool for researchers investigating the functions of Hepcidin in iron regulation dynamics and related disorders.
Product Name:
Rat Hepc (Hepcidin) ELISA Kit
SKU:
AEES00432
Target:
Rat Hepc (Hepcidin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Hepc. You can calculate the concentration of Rat Hepc in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
96-112
88-99
Average (%)
93
103
94
1:4
Range (%)
101-115
83-94
96-112
Average (%)
107
88
104
1:8
Range (%)
99-111
81-94
94-106
Average (%)
105
87
100
1:16
Range (%)
93-107
81-91
91-104
Average (%)
101
87
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
254.5
612.4
2443.0
232.2
578.0
2321.7
Standard deviation
13.5
29.4
80.6
14.2
31.2
90.5
C V (%)
5.3
4.8
3.3
6.12
5.4
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Hepc concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Hepc in samples. No significant cross-reactivity or interference between Rat Hepc and analogues was observed.
