The Rat Hedgehog Homolog Sonic (SHH) ELISA Kit is specifically designed for the precise measurement of Sonic hedgehog (SHH) levels in rat serum, plasma, and cell culture supernatants. This ELISA kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SHH is a key signaling protein involved in embryonic development, cell growth, and tissue repair. Dysregulation of the SHH pathway has been linked to various diseases including cancers, developmental disorders, and neurological conditions.
By detecting and quantifying SHH levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat Hedgehog Homolog Sonic (SHH) ELISA Kit is a powerful tool for investigating the role of SHH in biological processes and disease pathology, making it a valuable addition to any research laboratory.
Product Name:
Rat Hedgehog Homolog, Sonic (SHH) ELISA Kit
Product Code:
RTDL00953
Size:
96 Assays
Target:
SHH
Synonyms:
HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
