Rat Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
SKU:
RTDL00513
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P63039
Sensitivity:
1.27ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
HSP60,GROEL,CPN60,HuCHA60,Heat Shock Protein 60,Spastic Paraplegia 13,Autosomal Dominant,60 kDa chaperonin,Mitochondrial matrix protein P1,P62 lymphocyte protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Rat Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
The Rat Heat Shock 60KD Protein 1 (Chaperonin HSPD1) ELISA Kit is specifically designed for the precise quantification of HSPD1 levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.HSPD1, also known as heat shock protein 60 (HSP60), is a vital chaperonin protein involved in protein folding and cellular stress response. It plays a crucial role in maintaining cellular homeostasis and protecting cells from damage. Aberrant HSPD1 levels have been linked to various diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer, highlighting its importance as a potential biomarker for disease research and therapeutic development.
The Rat Heat Shock 60KD Protein 1 (Chaperonin HSPD1) ELISA Kit provides researchers with a valuable tool for studying the function and regulation of HSPD1 in rat models, offering insights into its role in health and disease. Order this kit today to advance your research in the field of protein biology and cellular stress response.
Product Name:
Rat Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
Product Code:
RTDL00513
Size:
96 Assays
Target:
HSPD1
Synonyms:
HSP60, GROEL, CPN60, HuCHA60, Heat Shock Protein 60, Spastic Paraplegia 13,Autosomal Dominant, 60 kDa chaperonin, Mitochondrial matrix protein P1, P60 lymphocyte protein
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
