The Rat HDL (High Density Lipoprotein) ELISA Kit is specifically designed to quantitatively detect and measure the levels of High Density Lipoprotein, a critical component in the lipid transport system. High Density Lipoprotein is often referred to as the "good" cholesterol due to its role in transporting cholesterol away from the arteries to the liver for processing and elimination. As a key player in cholesterol metabolism, HDL helps reduce the risk of cardiovascular disease by acting as an anti-atherogenic agent.
Accurate measurement of High Density Lipoprotein levels is vital for assessing lipid metabolism and cardiovascular health. The Rat HDL ELISA Kit provides excellent sensitivity and specificity for precise and reproducible results, making it an invaluable tool for researchers studying lipid profiles, cardiovascular diseases, and lipid-related disorders. Manufactured under strict quality control standards, this kit ensures robust performance and ease of use, facilitating comprehensive investigations into the role of HDL in maintaining overall health and preventing cardiovascular complications.
Product Name:
Rat HDL (High Density Lipoprotein) ELISA Kit
SKU:
AEES00434
Target:
Rat HDL (High Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HDL. You can calculate the concentration of Rat HDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
84-98
96-112
Average (%)
103
91
102
1:4
Range (%)
87-101
84-95
88-100
Average (%)
92
88
93
1:8
Range (%)
91-103
79-94
88-104
Average (%)
98
86
95
1:16
Range (%)
88-100
82-96
86-96
Average (%)
94
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.72
19.1
68.27
9.69
17.42
70.91
Standard deviation
0.65
0.86
2.38
0.59
0.97
3.79
C V (%)
6.69
4.5
3.49
6.09
5.57
5.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat HDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HDL in samples. No significant cross-reactivity or interference between Rat HDL and analogues was observed.
