The Rat HDL (High-Density Lipoprotein) CLIA Kit is a high-quality assay kit designed for the precise measurement of HDL levels in rat serum, plasma, and other biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for your research needs.HDL, also known as "good" cholesterol, plays a vital role in lipid metabolism and cardiovascular health. Monitoring HDL levels is crucial in understanding the risk of heart disease and other related conditions, making this kit an essential tool for researchers studying lipid disorders and cardiovascular health.
With easy-to-follow instructions and quick assay procedures, the Rat HDL CLIA Kit is a reliable solution for detecting and quantifying HDL levels in rat samples. Trust in this kit to provide you with precise and reliable data for your research studies.
Product Name:
Rat HDL (High Density Lipoprotein) CLIA Kit
SKU:
RTES00298
Target:
Rat HDL (High Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HDL. You can calculate the concentration of Rat HDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-104
90-103
Average (%)
104
96
96
1:4
Range (%)
101-113
89-102
88-99
Average (%)
107
95
94
1:8
Range (%)
91-106
99-113
89-101
Average (%)
98
104
94
1:16
Range (%)
87-100
86-99
101-117
Average (%)
93
92
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
93-109
99
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.85
5.84
16.75
2.01
5.93
16.94
Standard deviation
0.16
0.44
1.08
0.17
0.45
1.16
C V (%)
8.65
7.53
6.45
8.46
7.59
6.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HDL in samples. No significant cross-reactivity or interference between Rat HDL and analogues was observed.
