The Rat γGT1 (Gamma Glutamyltransferase 1) ELISA Kit is a specialized assay designed for the precise quantification of Gamma Glutamyltransferase 1 levels in rat samples. Gamma Glutamyltransferase 1, an important enzyme involved in various cellular processes, plays a critical role in the metabolism and turnover of glutathione, a key antioxidant molecule. Studies have linked Gamma Glutamyltransferase 1 to liver health, oxidative stress, and drug metabolism, highlighting its significance in maintaining cellular homeostasis.
Accurate measurement of Gamma Glutamyltransferase 1 levels using the Rat γGT1 ELISA Kit can provide valuable insights into liver function, oxidative balance, and potential biomarkers for liver diseases and other pathologies. Understanding the role of Gamma Glutamyltransferase 1 in disease mechanisms and cellular function is essential in biomedical research and clinical diagnostics. With exceptional sensitivity and specificity, the Rat γGT1 ELISA Kit ensures precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research studies focusing on liver biology, antioxidant defense, and related fields.
Product Name:
Rat γGT1 (Gamma Glutamyltransferase 1) ELISA Kit
SKU:
AEES00405
Target:
Rat γGT1 (Gamma Glutamyltransferase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat γGT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat γGT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat γGT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat γGT1. You can calculate the concentration of Rat γGT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
94-105
92-104
Average (%)
96
99
98
1:4
Range (%)
93-107
87-98
83-94
Average (%)
100
93
87
1:8
Range (%)
87-99
85-97
82-94
Average (%)
93
90
88
1:16
Range (%)
94-108
80-91
88-101
Average (%)
99
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.81
9.78
41.87
4.36
9.54
44.98
Standard deviation
0.3
0.48
1.98
0.28
0.39
1.41
C V (%)
6.24
4.91
4.73
6.42
4.09
3.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat γGT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat γGT1 in samples. No significant cross-reactivity or interference between Rat γGT1 and analogues was observed.
