The Rat GT (Gastrin) ELISA Kit is a specialized assay designed for the precise quantification of Gastrin levels in various biological samples obtained from rats. Gastrin is an essential peptide hormone that plays a crucial role in stimulating gastric acid secretion and regulating gastrointestinal functions, including gastric motility and mucosal growth.
Its functions extend to the modulation of digestive processes and interaction with other hormones in the gastrointestinal tract. This ELISA kit provides researchers with a reliable and accurate method to measure Gastrin concentrations, enabling a better understanding of its role in regulating gastric acid production, gut motility, and mucosal integrity. By accurately determining Gastrin levels, researchers can explore the hormone's involvement in various physiological processes, including digestion, and its implications in gastrointestinal disorders and diseases. Manufactured under strict quality control standards, our Rat GT ELISA Kit ensures robust performance and delivers reproducible results. The exceptional sensitivity and specificity of this kit make it a valuable tool for research studies focusing on Gastrin's functions and its interactions within the gastrointestinal system. Its user-friendly design further simplifies the assay procedure, making it an excellent choice for researchers investigating the physiological roles of Gastrin in gastrointestinal health and disease.
Product Name:
Rat GT (Gastrin) ELISA Kit
SKU:
AEES00422
Target:
Rat GT (Gastrin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GT. During the reaction, GT in the sample or standard competes with a fixed amount of GT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
87-103
94-109
Average (%)
99
94
100
1:4
Range (%)
88-102
87-103
100-115
Average (%)
94
94
106
1:8
Range (%)
82-96
91-107
99-114
Average (%)
89
98
106
1:16
Range (%)
89-104
88-99
95-108
Average (%)
95
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.3
392.1
1452.7
185.2
368.8
1344.2
Standard deviation
10.5
17.6
69.7
9.6
15.9
57.8
C V (%)
5.58
4.49
4.8
5.18
4.31
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GT in samples. No significant cross-reactivity or interference between GT and analogues was observed.
