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Rat Growth Regulated Oncogene Beta (GROb) ELISA Kit

SKU:
RTDL00462
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30348
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL2,SCYB2,MIP2,GRO2,MIP2a,MGSAb,CINC2a,HSF,Macrophage inflammatory protein 2-alpha,Hematopoietic synergistic factor,Chemokine(C-X-C-Motif)ligand 2
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Rat Growth Regulated Oncogene Beta (GROb) ELISA Kit

The Rat Growth Regulated Oncogene Beta (GROb) ELISA Kit is designed for the accurate detection of GROb levels in rat serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying the role of GROb in various physiological processes. GROb, also known as CXCL2, is a chemokine involved in inflammation, immune response, and cell migration.

It has been implicated in numerous disease states, including cancer, inflammatory disorders, and autoimmune diseases. The ability to accurately measure GROb levels using this ELISA kit makes it an invaluable tool for understanding the role of GROb in these conditions and developing potential therapeutic interventions.

Product Name:Rat Growth Regulated Oncogene Beta (GROb) ELISA Kit
Product Code:RTDL00462
Size:96 Assays
Target:GROb
Synonyms:CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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