Rat GROγ/CXCL3 (Growth Regulated Oncogene Gamma) ELISA Kit
- SKU:
- AEES00500
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat GROγ/CXCL3 (Growth Regulated Oncogene Gamma) ELISA Kit
The Rat GROγ/CXCL3 (Growth Regulated Oncogene Gamma) ELISA Kit is expertly developed for the precise quantitative assessment of GROγ/CXCL3 levels in diverse rat biological samples. GROγ, also known as CXCL3, belongs to the CXC chemokine family and plays a crucial role in inflammation, immune response modulation, and cancer biology.
As a potent chemoattractant, GROγ/CXCL3 contributes to the regulation of cell migration, angiogenesis, and the recruitment of immune cells to sites of injury or infection. Accurate measurement of GROγ/CXCL3 levels using this ELISA kit enables researchers to delve into the intricate roles of this chemokine in various physiological and pathological processes. Understanding the involvement of GROγ/CXCL3 in inflammatory responses, tumorigenesis, and immune regulation is paramount for elucidating its significance as a potential biomarker and therapeutic target in conditions related to inflammation and cancer. The Rat GROγ/CXCL3 ELISA Kit by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing dependable and reproducible data. With stringent quality control measures in place, this kit delivers robust performance and user-friendly operation, making it an ideal choice for research aiming to unravel the complexities of GROγ/CXCL3 biology and its implications for health and disease.
| Product Name: | Rat GROγ/CXCL3 (Growth Regulated Oncogene Gamma) ELISA Kit |
| Product Code: | AEES00500 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat GROγ/CXCL3 in samples. No significant cross-reactivity or interference between Rat GROγ/CXCL3 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat GROγ/CXCL3 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GROγ/CXCL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GROγ/CXCL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROγ/CXCL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GROγ/CXCL3. You can calculate the concentration of Rat GROγ/CXCL3 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GROγ/CXCL3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GROγ/CXCL3 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 95.10 | 193.80 | 913.50 | 90.90 | 189.10 | 838.30 |
| Standard deviation | 5.50 | 8.50 | 34.70 | 6.20 | 7.90 | 44.40 |
| CV (%) | 5.78 | 4.39 | 3.80 | 6.82 | 4.18 | 5.30 |
Recovery
The recovery of Rat GROγ/CXCL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-106 | 101 |
| EDTA plasma (n=8) | 90-104 | 96 |
| Cell culture media (n=8) | 95-106 | 100 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
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