Rat GROα/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
The Rat GROα/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit is a specialized assay designed for the quantitative detection of GROα/CXCL1 levels in rat biological samples.
GROα, also known as CXCL1, belongs to the CXC chemokine family and plays a vital role in immune responses, inflammation, and cancer development. This ELISA kit offers exceptional sensitivity and specificity, allowing precise measurement of GROα/CXCL1 concentrations in rat samples. The accurate detection of GROα/CXCL1 is crucial for research focusing on inflammatory responses, tumor microenvironments, and immune regulation, providing valuable insights into various pathological conditions. Manufactured under stringent quality control standards, this ELISA kit ensures robust performance, reliable results, and ease of use, making it an excellent choice for researchers investigating the functions and therapeutic implications of GROα/CXCL1 in biological systems.
Product Name:
Rat GROα/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
SKU:
AEES00287
Target:
Rat GROα/CXCL1 (Growth Regulated Oncogene Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GROα/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GROα/CXCL1. You can calculate the concentration of Rat GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
96-110
89-106
Average (%)
103
104
97
1:4
Range (%)
96-109
81-95
93-107
Average (%)
103
87
101
1:8
Range (%)
95-111
83-92
92-108
Average (%)
101
88
99
1:16
Range (%)
97-112
81-95
91-108
Average (%)
104
87
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.2
114.1
499.8
56.1
103.6
545.5
Standard deviation
3.3
5.7
20.5
3.3
4.4
26.7
C V (%)
6.32
5.0
4.1
5.88
4.25
4.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GROα/CXCL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GROα/CXCL1 in samples. No significant cross-reactivity or interference between Rat GROα/CXCL1 and analogues was observed.
