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Rat Granulin (GRN) ELISA Kit

SKU:
RTDL00461
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P23785
Sensitivity:
0.47ng/mL
Range:
1.25-80ng/mL
ELISA Type:
Sandwich
Synonyms:
GEP,GP88,PCDGF,PEPI,PGRN,Proepithelin,Acrogranin,Glycoprotein of 88 Kda,Paragranulin
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Granulin (GRN) ELISA Kit

The Rat Granulin (GRN) ELISA Kit is specifically designed for the precise measurement of granulin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Granulin is a vital protein that plays a role in cell growth, tissue repair, and inflammation. Dysregulation of granulin has been linked to various diseases such as Alzheimer's, frontotemporal dementia, and cancer, highlighting its importance as a biomarker for studying these conditions and exploring potential treatments.

Overall, the Rat Granulin (GRN) ELISA Kit is a valuable tool for researchers looking to investigate the role of granulin in disease pathogenesis and develop new therapeutic strategies.

Product Name:Rat Granulin (GRN) ELISA Kit
Product Code:RTDL00461
Size:96 Assays
Target:GRN
Synonyms:GEP, GP88, PCDGF, PEPI, PGRN, Proepithelin, Acrogranin, Glycoprotein of 88 Kda, Paragranulin
Detection Method:Sandwich
Range:1.25-80ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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