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Rat GR (Glucocorticoid Receptor) ELISA Kit

SKU:
AEES00409
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat GR (Glucocorticoid Receptor) ELISA Kit

The Rat GR (Glucocorticoid Receptor) ELISA Kit is a specialized assay designed for the quantitative analysis of glucocorticoid receptors in various rat biological samples. Glucocorticoid receptors are crucial transcription factors that play pivotal roles in cellular processes, including metabolism, inflammation, stress response, and immune modulation. As key regulators of gene expression, these receptors mediate the effects of glucocorticoid hormones on target cells, influencing a wide range of physiological functions. This ELISA kit provides researchers with a precise and reliable method to measure glucocorticoid receptor levels, offering valuable insights into the mechanisms of hormonal signaling, stress responses, and immune regulation.

Accurate quantification of glucocorticoid receptors is essential for understanding their involvement in numerous physiological pathways and their implications in various diseases and therapeutic interventions. With exceptional sensitivity and specificity, the Rat GR ELISA Kit from Assay Genie ensures reproducible and high-quality results. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it an ideal tool for research studying glucocorticoid receptor biology and related pathways.

Product Name:Rat GR (Glucocorticoid Receptor) ELISA Kit
Product Code:AEES00409
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat GR in samples. No significant cross-reactivity or interference between Rat GR and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat GR concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GR. You can calculate the concentration of Rat GR in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GR were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GR were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)0.530.854.430.540.924.75
Standard deviation0.030.040.200.040.050.23
CV (%)5.664.714.517.415.434.84

Recovery

The recovery of Rat GR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)96-111101
EDTA plasma (n=8)91-10498
Cell culture media (n=8)87-10093
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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