Rat GPT Recombinant Protein (RPPB1738)
- SKU:
- RPPB1738
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P25409
- Research Area:
- Enzymes
Description
| Product Name: | Rat GPT Recombinant Protein |
| Product Code: | RPPB1738 |
| Size: | 20µg |
| Species: | Rat |
| Target: | GPT |
| Synonyms: | Alanine aminotransferase 1 (EC:2.6.1.2), ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alaninetransaminase 1, Glutamic--pyruvic transaminase 1, Gpt, Aat1, Gpt1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GPT protein solution (1mg/ml) containing PhosphateBuffered Saline pH 7.4 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MGSMASRVND QSQASRNGLKGKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLALCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTMLKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVLCVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELASFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQAERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEETGICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS |
| Biological Activity: | Specific activity is> 60units/mg, and is defined as the amount of enzyme that cleaves 1umole ofL-Alanine to L-Glutamate per minute at pH 7.5 at 37C. |
GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.
GPT Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 519 amino acids (1-496 a.a) and having a molecular mass of 57.5kDa.�GPT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase Chromosomal Location of Human Ortholog: 7q34 Cellular Component: cytoplasm; extracellular space Molecular Function:L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding Biological Process: biosynthetic process; L-alanine catabolic process |
| NCBI Summary: | human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006] |
| UniProt Code: | P25409 |
| NCBI GenInfo Identifier: | 13591961 |
| NCBI Gene ID: | 81670 |
| NCBI Accession: | NP_112301.1 |
| UniProt Secondary Accession: | P25409,Q4V7F7, |
| UniProt Related Accession: | P25409 |
| Molecular Weight: | 55,110 Da |
| NCBI Full Name: | alanine aminotransferase 1 |
| NCBI Synonym Full Names: | glutamic--pyruvic transaminase |
| NCBI Official Symbol: | Gpt�� |
| NCBI Official Synonym Symbols: | ALAT; Gpt1�� |
| NCBI Protein Information: | alanine aminotransferase 1 |
| UniProt Protein Name: | Alanine aminotransferase 1 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1 |
| Protein Family: | NAD--protein ADP-ribosyltransferase |
| UniProt Gene Name: | Gpt�� |
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