The Rat GPC3 (Glypican 3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GPC3 levels in rat serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for various research applications.Glypican 3 is a cell surface protein that plays a critical role in cell growth, differentiation, and signaling pathways. Dysregulation of GPC3 expression has been linked to various diseases, including hepatocellular carcinoma and other cancers.
Therefore, the Rat GPC3 CLIA Kit is essential for studying the role of GPC3 in disease progression and identifying potential therapeutic targets.Overall, the Rat GPC3 CLIA Kit offers researchers a powerful tool for investigating the function and significance of Glypican 3 in rat models, ultimately advancing our understanding of disease mechanisms and potential treatment options.
Product Name:
Rat GPC3 (Glypican 3) CLIA Kit
SKU:
RTES00553
Target:
Rat GPC3 (Glypican 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GPC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GPC3. You can calculate the concentration of Rat GPC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
92-105
93-110
Average (%)
109
98
101
1:4
Range (%)
88-101
93-110
98-111
Average (%)
94
101
103
1:8
Range (%)
96-109
95-109
104-116
Average (%)
103
102
110
1:16
Range (%)
99-117
87-99
99-114
Average (%)
107
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.39
241.19
869.99
117.16
230.76
795.79
Standard deviation
11.16
23.93
89.61
11.52
18.81
53.64
C V (%)
10.2
9.92
10.3
9.83
8.15
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GPC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GPC3 in samples. No significant cross-reactivity or interference between Rat GPC3 and analogues was observed.
