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Rat Gonadotropin Releasing Hormone (GnRH) ELISA Kit

SKU:
RTDL00445
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07490
Sensitivity:
29pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
GNRH1,GRH,LNRH,LHRH,LH-RH I,Luliberin,Luteinizing-Hormone Releasing Hormone,Progonadoliberin-1,Gonadoliberin I,Gonadorelin,GnRH-associated peptide 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Gonadotropin Releasing Hormone (GnRH) ELISA Kit

The Rat Gonadotropin-Releasing Hormone (GnRH) ELISA Kit is a reliable tool for quantifying levels of GnRH in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for a variety of research applications.Gonadotropin-releasing hormone (GnRH) is a key regulator of reproduction in mammals, controlling the release of hormones that influence the maturation of reproductive tissues and the secretion of sex hormones.

Dysregulation of GnRH signaling is associated with reproductive disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.Overall, the Rat Gonadotropin-Releasing Hormone (GnRH) ELISA Kit provides researchers with a valuable tool for investigating the role of GnRH in reproductive health and developing new strategies for treating reproductive disorders in rats.

Product Name:Rat Gonadotropin Releasing Hormone (GnRH) ELISA Kit
Product Code:RTDL00445
Size:96 Assays
Target:GnRH
Synonyms:GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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