Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) ELISA Kit
The Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) ELISA Kit is a specialized assay designed for the quantitative detection of GnRHR levels in various biological samples from rats.
The gonadotropin-releasing hormone receptor, known for its essential role in reproductive physiology, is a key regulator of the hypothalamic-pituitary-gonadal axis. It is responsible for mediating the effects of gonadotropin-releasing hormone (GnRH), a pivotal hormone that controls the release of pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone (LH). This ELISA kit provides a precise and accurate method for quantifying GnRHR expression levels, offering important insights into the regulation of reproductive processes, including puberty onset, gonadal steroidogenesis, and fertility. By measuring GnRHR concentrations, researchers can deepen their understanding of the molecular mechanisms underlying reproductive function, hormone signaling pathways, and the pathophysiology of reproductive disorders. The Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, enabling reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, aiding in effortless analysis and making it an excellent choice for research in the fields of reproductive endocrinology, fertility, and hormonal regulation in rodents.
Product Name:
Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) ELISA Kit
SKU:
AEES00416
Target:
Rat GnRHR (Gonadotropin-Releasing Hormone Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GnRHR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GnRHR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GnRHR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GnRHR. You can calculate the concentration of Rat GnRHR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
94-108
92-105
Average (%)
105
99
98
1:4
Range (%)
87-100
84-95
86-98
Average (%)
94
89
91
1:8
Range (%)
93-104
79-94
86-99
Average (%)
98
86
92
1:16
Range (%)
87-101
86-99
82-94
Average (%)
93
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
90-107
98
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
2.54
8.8
1.08
2.42
9.1
Standard deviation
0.06
0.12
0.28
0.07
0.1
0.48
C V (%)
5.83
4.72
3.18
6.48
4.13
5.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GnRHR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GnRHR in samples. No significant cross-reactivity or interference between Rat GnRHR and analogues was observed.
