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Rat Glycine Dehydrogenase (GLDC) ELISA Kit

SKU:
RTDL00436
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D4A5Q9
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GCSP,NKH,Decarboxylating,Glycine Decarboxylase,Glycine Cleavage System Protein P,Glycine dehydrogenase (aminomethyl-transferring)
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Glycine Dehydrogenase (GLDC) ELISA Kit

The Rat Glycine Dehydrogenase (GLDC) ELISA Kit is a highly reliable and efficient tool for the detection of GLDC levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GLDC is an essential enzyme involved in glycine metabolism, playing a critical role in amino acid metabolism and neurotransmitter synthesis.

Dysregulation of GLDC has been linked to various metabolic disorders and neurological diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.By utilizing the Rat GLDC ELISA Kit, researchers can gain valuable insights into the role of GLDC in health and disease, advancing our understanding of metabolic processes and potential treatment strategies.

Product Name:Rat Glycine Dehydrogenase (GLDC) ELISA Kit
Product Code:RTDL00436
Size:96 Assays
Target:GLDC
Synonyms:GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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