Rat Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit
The Rat Glutamate Receptor Ionotropic N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit is a specialized assay designed for the accurate quantification of GRIN1 levels in rat samples, such as serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an invaluable tool for researchers studying the role of GRIN1 in neural function and neurological disorders.GRIN1 is a subunit of the NMDA receptor, a key player in synaptic plasticity and memory formation. Dysregulation of NMDA receptors has been implicated in various neurological conditions, including Alzheimer's disease, schizophrenia, and stroke.
By measuring GRIN1 levels, researchers can gain insights into the pathophysiology of these disorders and potentially identify new therapeutic targets.Overall, the Rat GRIN1 ELISA Kit is a powerful tool for investigating the role of NMDA receptors in neurological function and disease, providing valuable data for advancing our understanding of brain health and developing novel treatment strategies.
Product Name:
Rat Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
