The Rat GLUT4 (Glucose Transporter 4) ELISA Kit is a specialized assay designed for the quantitative detection of GLUT4 levels in various biological samples from rats. GLUT4, a key glucose transporter protein predominantly expressed in insulin-sensitive tissues such as adipose tissue and skeletal muscle, plays a critical role in glucose homeostasis by facilitating the uptake of glucose from the bloodstream into cells in response to insulin stimulation. Dysregulation of GLUT4 expression and activity can impact glucose metabolism, insulin sensitivity, and cellular energy balance, contributing to metabolic disorders like diabetes and obesity. This ELISA kit provides a reliable method for quantifying GLUT4 levels, offering valuable insights into its function in glucose uptake, insulin signaling, and metabolic regulation.
By accurately measuring GLUT4 concentrations, researchers can better understand the mechanisms underlying glucose transport, insulin responsiveness, and metabolic dysfunction related to various metabolic diseases and insulin resistance conditions. The Rat GLUT4 (Glucose Transporter 4) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, enabling reproducible and reliable results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for research studies investigating GLUT4's role in glucose metabolism, insulin action, and metabolic disorders.
Product Name:
Rat GLUT4 (Glucose Transporter 4) ELISA Kit
SKU:
AEES00414
Target:
Rat GLUT4 (Glucose Transporter 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLUT4. You can calculate the concentration of Rat GLUT4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
89-105
90-102
Average (%)
106
97
96
1:4
Range (%)
95-107
83-93
87-102
Average (%)
100
88
94
1:8
Range (%)
90-102
81-91
82-96
Average (%)
96
86
88
1:16
Range (%)
88-101
83-97
88-99
Average (%)
95
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.03
4.5
19.63
2.21
4.26
19.21
Standard deviation
0.11
0.21
0.98
0.13
0.18
0.75
C V (%)
5.42
4.67
4.99
5.88
4.23
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GLUT4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.
