The Rat GLUT2 (Glucose Transporter 2) ELISA Kit is engineered to quantitatively measure the levels of GLUT2 in rat biological samples. GLUT2, a critical facilitator of glucose transport across cellular membranes, plays a fundamental role in glucose homeostasis and metabolism regulation. GLUT2 is particularly expressed in tissues like liver, kidney, and pancreatic beta cells, contributing to glucose sensing, uptake, and secretion processes. Glucokinase, a key enzyme in the regulation of blood glucose levels, cooperates with GLUT2 in pancreatic beta cells and hepatocytes to modulate glucose sensing and insulin release.
Dysfunction in GLUT2 expression or activity is associated with impaired glucose metabolism, diabetes mellitus, and metabolic disorders. The Rat GLUT2 ELISA Kit provided by Assay Genie is tailored to deliver precise, sensitive, and specific measurements of GLUT2 levels, enabling researchers to explore the role of GLUT2 in glucose transport, cellular metabolism, and metabolic diseases. With stringent quality control measures in place, this kit ensures robust performance and reproducible results, making it an ideal tool for scientific investigations in diabetes research, metabolic studies, and related fields.
Product Name:
Rat GLUT2 (Glucose Transporter 2) ELISA Kit
SKU:
AEES00386
Target:
Rat GLUT2 (Glucose Transporter 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLUT2. You can calculate the concentration of Rat GLUT2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
98-110
95-109
Average (%)
92
104
102
1:4
Range (%)
93-108
86-97
84-98
Average (%)
99
91
91
1:8
Range (%)
91-103
81-95
85-99
Average (%)
98
86
91
1:16
Range (%)
85-97
82-95
85-98
Average (%)
92
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.11
8.8
1.17
2.08
9.62
Standard deviation
0.06
0.12
0.28
0.07
0.12
0.31
C V (%)
5.56
5.69
3.18
5.98
5.77
3.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GLUT2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GLUT2 in samples. No significant cross-reactivity or interference between Rat GLUT2 and analogues was observed.
