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Rat Glucose Transporter 2 (GLUT2) ELISA Kit (RTDL00441)

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SKU:
RTDL00441
Product Type:
ELISA Kit
Sensitivity:
0.33ng/mL
Range:
0.78-50ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC2A2,SLC2-A2,Solute carrier family 2, facilitated glucose transporter member 2,Glucose transporter type 2, liver
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Rat Glucose Transporter 2 (GLUT2) ELISA Kit

The Rat GLUT2 (Glucose Transporter 2) ELISA Kit is a reliable and accurate tool for detecting levels of GLUT2 in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for various research applications.GLUT2 is a key protein responsible for glucose transport across cell membranes, playing a crucial role in maintaining glucose homeostasis in the body.

Dysregulation of GLUT2 expression has been implicated in metabolic disorders such as diabetes, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Rat GLUT2 ELISA Kit is a valuable asset for researchers interested in understanding the role of GLUT2 in metabolic regulation and exploring its potential implications for various health conditions.

Product Name:Rat Glucose Transporter 2 (GLUT2) ELISA Kit
Product Code:RTDL00441
Size:96 Assays
Target:GLUT2
Synonyms:SLC2A2, SLC2-A2, Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver
Detection Method:Sandwich
Range:0.78-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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