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Rat Glucagon (GC) ELISA Kit

SKU:
RTDL00415
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06883
Sensitivity:
10.8pg/mL
Range:
23.437-1500pg/mL
ELISA Type:
Competitive
Synonyms:
GCG,GLP1,GLP2,GRPP,Glicentin-Related Polypeptide,Glucagen,Oxyntomodulin,Incretin hormone
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Glucagon (GC) ELISA Kit

The Rat Glucagon (GC) ELISA Kit is a reliable tool for the precise measurement of glucagon levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it perfect for various research applications.Glucagon is a key hormone that regulates blood glucose levels by promoting the release of glucose from the liver.

It plays an essential role in metabolic processes and is implicated in conditions such as diabetes and metabolic disorders. Monitoring glucagon levels can provide valuable insights into these diseases and aid in the development of targeted therapies.Overall, the Rat Glucagon (GC) ELISA Kit offers a valuable tool for researchers studying metabolic disorders and exploring potential treatment options.

Product Name:Rat Glucagon (GC) ELISA Kit
Product Code:RTDL00415
Size:96 Assays
Target:GC
Synonyms:GCG, GLP1, GLP2, GRPP, Glicentin-Related Polypeptide, Glucagen, Oxyntomodulin, Incretin hormone
Detection Method:Competition
Range:23.437-1500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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