The Rat Glucagon Like Peptide 1 (GLP-1) ELISA Kit is a specialized assay meticulously crafted for the quantitative detection of GLP-1 levels in rat biological samples. GLP-1, a crucial hormone primarily produced in the intestines, plays a pivotal role in glucose homeostasis, insulin release, and appetite regulation. This ELISA kit provides researchers with a versatile tool to explore the dynamic functions of GLP-1 in metabolic processes and its possible implications in various physiological functions. Accurate measurement of GLP-1 levels is essential for unraveling its intricate involvement in glucose metabolism, insulin secretion, and energy balance.
With the Rat GLP-1 ELISA Kit, researchers can delve deeper into the intricate mechanisms governed by GLP-1, shedding light on its therapeutic potential in metabolic disorders and diabetes research. Engineered with high sensitivity and specificity, this ELISA kit ensures the generation of robust and reproducible results, empowering researchers to uncover novel insights into the regulatory roles of GLP-1 in metabolic pathways. Trust in the accuracy and reliability of the Rat GLP-1 ELISA Kit to propel your research forward and enhance discoveries in the domain of metabolic physiology and endocrine regulation.
Product Name:
Rat GLP-1 (Glucagon Like Peptide 1) ELISA Kit
SKU:
AEES00523
Target:
Rat GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
101-110
89-98
Average (%)
95
104
93
1:4
Range (%)
94-102
98-110
85-95
Average (%)
99
103
90
1:8
Range (%)
84-97
98-111
88-95
Average (%)
90
104
92
1:16
Range (%)
89-97
87-101
99-108
Average (%)
94
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-103
99
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
83-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.19
10.25
48.72
4.77
10.96
47.45
Standard deviation
0.23
0.5
2.43
0.26
0.52
2.43
C V (%)
5.49
4.88
4.99
5.45
4.74
5.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GLP-1 in samples. No significant cross-reactivity or interference between Rat GLP-1 and analogues was observed.
