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Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit (AEES00406)

SKU:
AEES00406
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.38 ng/mL
Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
Frequently bought together:

Description

Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit

The Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit is a precision assay tailored for the accurate detection and quantification of Gastric Inhibitory Polypeptide in rat biological samples. Gastric Inhibitory Polypeptide, also known as glucose-dependent insulinotropic peptide, is a crucial hormone secreted by the intestinal K-cells in response to food intake. It plays a significant role in regulating blood sugar levels and insulin release postprandially, making it essential for metabolic control and energy balance. Measuring GIP levels using the Rat GIP ELISA Kit enables researchers to gain insights into glucose metabolism, appetite regulation, and hormonal responses to nutrient ingestion.

Understanding the role of Gastric Inhibitory Polypeptide in metabolic processes and its potential implications in metabolic disorders is vital for advancing research in diabetes, obesity, and related fields. Delivering exceptional sensitivity and specificity, the Rat GIP ELISA Kit ensures precise and reproducible results. Manufactured under strict quality standards, this kit guarantees robust performance and straightforward protocols, making it an ideal choice for investigations in the fields of endocrinology, metabolism, and diabetes research.

Product Name:Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit
Product Code:AEES00406
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.63-40 ng/mL
Sensitivity:0.38 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat GIP in samples. No significant cross-reactivity or interference between Rat GIP and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat GIP concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GIP. You can calculate the concentration of Rat GIP in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GIP were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GIP were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)2.004.1119.562.083.8818.52
Standard deviation0.130.240.710.130.200.93
CV (%)6.505.843.636.255.155.02

Recovery

The recovery of Rat GIP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)93-106100
EDTA plasma (n=8)91-10498
Cell culture media (n=8)86-9993
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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