Rat GHRH (Growth Hormone Releasing Hormone) ELISA Kit
The Rat GHRH (Growth Hormone Releasing Hormone) ELISA Kit is a specialized assay designed to precisely quantify the levels of GHRH in various biological samples obtained from rats. GHRH is a vital neuropeptide that plays a pivotal role in regulating the synthesis and release of growth hormone from the pituitary gland. Its functions include stimulating growth, cell regeneration, and metabolism, making it a key player in the endocrine system. This ELISA kit provides researchers with a reliable and sensitive tool to measure GHRH concentrations, enabling a better understanding of the mechanisms involved in growth hormone regulation and its impact on growth and development processes. By accurately determining GHRH levels, researchers can gain insights into the dynamics of hormonal regulation, particularly in relation to growth-related processes, metabolic functions, and potential implications in various physiological and pathological conditions.
Manufactured with stringent quality control measures, our Rat GHRH ELISA Kit ensures robust and reproducible performance, allowing for accurate and consistent results. The exceptional sensitivity and specificity of this kit make it a valuable asset for researchers conducting studies on the role of GHRH in growth regulation, metabolism, and other physiological processes. Its ease of use further enhances the efficiency and productivity of research endeavors, making it an excellent choice for investigations in endocrinology and related fields.
Product Name:
Rat GHRH (Growth Hormone Releasing Hormone) ELISA Kit
SKU:
AEES00421
Target:
Rat GHRH (Growth Hormone Releasing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GHRH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GHRH. You can calculate the concentration of Rat GHRH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
97-110
92-106
Average (%)
95
105
97
1:4
Range (%)
95-108
83-96
87-99
Average (%)
100
88
93
1:8
Range (%)
85-99
83-93
81-95
Average (%)
92
88
87
1:16
Range (%)
91-103
84-97
81-92
Average (%)
97
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.14
102.24
400.64
45.74
104.25
431.33
Standard deviation
3.32
5.84
20.67
2.98
5.58
19.93
C V (%)
6.76
5.71
5.16
6.52
5.35
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GHRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GHRH in samples. No significant cross-reactivity or interference between Rat GHRH and analogues was observed.
