Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
The Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit is specifically designed for the quantitative detection of GFAP levels in rat serum, plasma, and tissue homogenates. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for your research needs.GFAP is a key protein found in astrocytes, the most abundant cells in the central nervous system. Changes in GFAP levels are associated with various neurological disorders, including traumatic brain injury, Alzheimer's disease, and multiple sclerosis.
By measuring GFAP levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Whether you are studying neurodegenerative diseases or investigating the impact of brain injuries, the Rat GFAP ELISA Kit from Assay Genie is a valuable tool for your research. Get reliable and reproducible results to advance your understanding of GFAP biology and its implications in neurological disorders.
Product Name:
Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
SKU:
RTES00998
Target:
Rat GFAP (Glial Fibrillary Acidic Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GFAP. You can calculate the concentration of Rat GFAP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
94-107
85-100
Average (%)
105
101
92
1:4
Range (%)
87-101
80-91
83-96
Average (%)
94
86
88
1:8
Range (%)
90-104
81-92
84-96
Average (%)
95
87
90
1:16
Range (%)
86-100
80-93
90-100
Average (%)
93
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
2.05
9.71
1.01
2.17
10.47
Standard deviation
0.06
0.12
0.38
0.06
0.13
0.52
C V (%)
5.83
5.85
3.91
5.94
5.99
4.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GFAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GFAP in samples. No significant cross-reactivity or interference between Rat GFAP and analogues was observed.
