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Rat GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit

SKU:
AEES00415
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.38 ng/mL
Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit

The Rat GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit is a specialized assay designed for the quantitative detection of GDH/GLDH levels in various biological samples from rats. GDH/GLDH is a critical enzyme involved in amino acid metabolism, specifically the conversion of glutamate to alpha-ketoglutarate with the concomitant reduction of NAD(P)+ to NAD(P)H.

This enzyme plays a pivotal role in the tricarboxylic acid cycle (TCA) and contributes to the regulation of cellular energy production and nitrogen balance. This ELISA kit provides a precise and accurate method for quantifying GDH/GLDH levels, offering valuable insights into its function in amino acid metabolism, energy generation, and overall cellular metabolic processes. By measuring GDH/GLDH concentrations, researchers can gain a better understanding of its role in metabolic pathways, cellular homeostasis, and the pathogenesis of various metabolic disorders and diseases. The Rat GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, allowing for reliable and reproducible results. Manufactured under strict quality control measures, this kit delivers robust performance, facilitating ease of use and analysis, and making it an excellent choice for research in the fields of amino acid metabolism, cellular energetics, and metabolic disorders.

Product Name:Rat GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit
Product Code:AEES00415
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.63-40 ng/mL
Sensitivity:0.38 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat GDH/GLDH in samples. No significant cross-reactivity or interference between Rat GDH/GLDH and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat GDH/GLDH concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GDH/GLDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GDH/GLDH. You can calculate the concentration of Rat GDH/GLDH in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GDH/GLDH were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GDH/GLDH were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)2.135.9718.102.166.5019.46
Standard deviation0.120.340.710.120.330.85
CV (%)5.635.703.925.565.084.37

Recovery

The recovery of Rat GDH/GLDH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)91-10496
EDTA plasma (n=8)93-108101
Cell culture media (n=8)89-10196
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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