Rat GDF5 (Growth Differentiation Factor 5) CLIA Kit
The Rat GDF5 (Growth Differentiation Factor 5) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of GDF5 levels in rat samples, including serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it a valuable tool for studying the role of GDF5 in various biological processes.GDF5 is a member of the bone morphogenetic protein family and is involved in regulating growth and differentiation of various cell types, including cartilage and bone cells.
It plays a crucial role in skeletal development and joint formation, making it a key target for research in musculoskeletal disorders such as osteoarthritis and osteoporosis.By accurately measuring GDF5 levels in rat samples, researchers can gain valuable insights into the mechanisms underlying these conditions and identify potential therapeutic targets. The Rat GDF5 CLIA Kit is a reliable and effective tool for studying the role of GDF5 in skeletal development and disease progression.
Product Name:
Rat GDF5 (Growth Differentiation Factor 5) CLIA Kit
SKU:
RTES00267
Target:
Rat GDF5 (Growth Differentiation Factor 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF5. You can calculate the concentration of Rat GDF5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
85-98
100-118
Average (%)
108
91
108
1:4
Range (%)
93-109
101-115
102-116
Average (%)
101
109
109
1:8
Range (%)
104-119
94-111
87-97
Average (%)
110
101
92
1:16
Range (%)
85-99
92-105
104-120
Average (%)
92
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.18
236.74
846.55
104.33
235.03
790.14
Standard deviation
11.6
22.66
54.35
13.0
27.29
78.07
C V (%)
11.03
9.57
6.42
12.46
11.61
9.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF5 in samples. No significant cross-reactivity or interference between Rat GDF5 and analogues was observed.
