Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit
The Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit is a powerful tool for accurately measuring levels of GDF3 in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.GDF3 is a key protein involved in growth and differentiation processes, playing a pivotal role in developmental pathways and cellular functions.
Its dysregulation has been linked to various diseases and disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.With the Rat GDF3 CLIA Kit, researchers can confidently study the function and dynamics of GDF3 in rat models, paving the way for new insights and advancements in the field of molecular biology and disease research.
Product Name:
Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit
SKU:
RTES00266
Target:
Rat GDF3 (Growth Differentiation Factor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF3. You can calculate the concentration of Rat GDF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
92-106
84-96
Average (%)
106
98
91
1:4
Range (%)
86-101
87-99
97-111
Average (%)
93
93
104
1:8
Range (%)
99-117
100-115
93-109
Average (%)
107
108
100
1:16
Range (%)
84-95
94-107
95-108
Average (%)
90
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.85
155.14
359.01
54.47
143.59
376.44
Standard deviation
4.35
17.14
22.01
4.42
14.65
41.94
C V (%)
8.73
11.05
6.13
8.11
10.2
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF3 in samples. No significant cross-reactivity or interference between Rat GDF3 and analogues was observed.
