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Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit

SKU:
AEES00420
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
Frequently bought together:

Description

Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit

The Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit is expertly designed to facilitate the precise and quantitative measurement of GDF11 levels across a variety of biological samples collected from rats. GDF11, a member of the TGF-beta superfamily, is a critical regulator of cell growth, differentiation, and development, playing a vital role in multiple physiological processes such as embryogenesis, tissue regeneration, and organ maintenance. Its actions are particularly important in skeletal muscle and cardiovascular tissues, where GDF11 contributes to maintaining tissue homeostasis and proper functioning. This ELISA kit provides researchers with a highly sensitive and specific method to quantify GDF11 levels, offering valuable insights into the regulatory roles of this growth factor in various biological systems.

By accurately measuring GDF11 concentrations, scientists can decipher its impact on cellular differentiation, tissue growth, and potential implications in conditions related to muscle function, cardiovascular health, and aging. Our Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit is manufactured according to stringent quality standards, ensuring robust and reliable performance. With exceptional sensitivity and specificity, this kit enables researchers to generate accurate and reproducible results, making it an indispensable tool for investigating the intricate functions of GDF11 in developmental processes and tissue maintenance. Its user-friendly design further enhances its utility in a wide range of research applications focused on understanding the role of GDF11 in various biological contexts.

Product Name:Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit
Product Code:AEES00420
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat GDF11 in samples. No significant cross-reactivity or interference between Rat GDF11 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat GDF11 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GDF11. You can calculate the concentration of Rat GDF11 in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GDF11 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GDF11 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)53.4598.60337.4048.54104.30358.16
Standard deviation3.404.1310.263.326.1318.91
CV (%)6.364.193.046.845.885.28

Recovery

The recovery of Rat GDF11 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)87-10094
EDTA plasma (n=8)94-109100
Cell culture media (n=8)84-9991
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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