Rat GCK (Glucokinase) ELISA Kit (AEES00412)
- SKU:
- AEES00412
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.38 ng/mL
- Range:
- 0.63-40 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat GCK (Glucokinase) ELISA Kit
The Rat GCK (Glucokinase) ELISA Kit is a specialized assay tailored to quantitatively measure levels of glucokinase, a key enzyme involved in glucose metabolism, in various rat biological samples. Glucokinase plays a vital role in regulating glucose homeostasis by facilitating glucose phosphorylation in the liver and pancreatic beta cells, contributing to glucose sensing, insulin secretion, and overall glucose utilization.
Dysregulation of glucokinase activity can lead to metabolic imbalances and impact glucose control, potentially influencing the development of metabolic disorders like diabetes. This ELISA kit offers a reliable and accurate method for assessing glucokinase levels in biological specimens, providing valuable insights into the enzyme's involvement in glucose metabolism, insulin sensitivity, and metabolic health. By quantifying glucokinase concentrations, researchers can better understand its role in glucose utilization and its implications in metabolic disorders, paving the way for targeted therapeutic interventions and potential strategies for managing metabolic conditions. The Rat GCK (Glucokinase) ELISA Kit from Assay Genie delivers exceptional sensitivity and specificity, ensuring precise and reproducible results. Produced under strict quality control measures, this kit ensures robust performance and user-friendly protocols, making it an ideal choice for researchers investigating the critical role of glucokinase in glucose metabolism and its impact on metabolic health and disease pathogenesis.
| Product Name: | Rat GCK (Glucokinase) ELISA Kit |
| Product Code: | AEES00412 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.63-40 ng/mL |
| Sensitivity: | 0.38 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat GCK in samples. No significant cross-reactivity or interference between Rat GCK and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat GCK concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GCK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GCK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GCK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GCK. You can calculate the concentration of Rat GCK in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
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