The Rat GATA4 (GATA Binding Protein 4) CLIA Kit is a reliable and accurate assay designed for the quantification of GATA4 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for researchers in a variety of fields.GATA4 is a transcription factor that plays a crucial role in the regulation of gene expression, particularly in the development and function of the heart and other tissues.
Dysregulation of GATA4 is associated with various cardiovascular diseases and congenital heart defects, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat GATA4 CLIA Kit provides researchers with a powerful tool for investigating the role of GATA4 in health and disease, ultimately contributing to advancements in cardiovascular research and the development of novel treatment strategies.
Product Name:
Rat GATA4 (GATA Binding Protein 4) CLIA Kit
SKU:
RTES00234
Target:
Rat GATA4 (GATA Binding Protein 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GATA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GATA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GATA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GATA4. You can calculate the concentration of Rat GATA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
101-117
84-99
Average (%)
107
109
90
1:4
Range (%)
94-107
98-115
95-110
Average (%)
100
105
100
1:8
Range (%)
86-98
86-96
84-98
Average (%)
93
91
91
1:16
Range (%)
97-111
88-104
86-99
Average (%)
103
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
208.75
555.99
1341.72
192.01
561.83
1305.56
Standard deviation
22.06
56.32
150.54
18.74
42.92
116.06
C V (%)
10.57
10.13
11.22
9.76
7.64
8.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GATA4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GATA4 in samples. No significant cross-reactivity or interference between Rat GATA4 and analogues was observed.
