The Rat GATA3 (GATA Binding Protein 3) CLIA Kit is specifically designed for the quantitative detection of GATA3 levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.GATA3 is a key transcription factor that plays a critical role in the regulation of gene expression and cell differentiation. It is involved in various physiological processes, including immune response, T-cell development, and cancer progression.
Therefore, accurate measurement of GATA3 levels can provide valuable insights into these processes and may help in the development of novel therapeutic strategies.This CLIA kit is easy to use and provides rapid results, making it an excellent tool for researchers studying GATA3-related pathways and diseases in rat models. Trust in the reliability of this kit to support your research efforts and advance scientific knowledge in this field.
Product Name:
Rat GATA3 (GATA Binding Protein 3) CLIA Kit
SKU:
RTES00233
Target:
Rat GATA3 (GATA Binding Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GATA3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GATA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GATA3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GATA3. You can calculate the concentration of Rat GATA3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
98-114
91-103
Average (%)
107
105
96
1:4
Range (%)
95-108
99-115
93-107
Average (%)
102
107
99
1:8
Range (%)
102-118
94-104
100-115
Average (%)
109
99
107
1:16
Range (%)
103-117
91-107
102-115
Average (%)
109
98
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
88-102
96
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.44
95.18
288.84
44.28
98.99
274.41
Standard deviation
5.25
11.33
19.61
5.49
8.25
27.14
C V (%)
12.67
11.9
6.79
12.4
8.33
9.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GATA3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GATA3 in samples. No significant cross-reactivity or interference between Rat GATA3 and analogues was observed.
