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Rat Galectin 1 (GAL1) ELISA Kit

SKU:
RTDL00406
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11762
Sensitivity:
0.246ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS1,GBP,HLBP14,Galaptin,Lectin,Galactoside-Binding Soluble 1,14 kDa laminin-binding protein,Lactose-binding lectin 1,Putative MAPK-activating protein PM12,S-Lac lectin
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Rat Galectin 1 (GAL1) ELISA Kit

The Rat Galectin-1 (GAL1) ELISA Kit is specifically designed to detect levels of Galectin-1 in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Galectin-1 is a protein known for its role in modulating immune responses, cell adhesion, and apoptosis. It is also implicated in various diseases, including cancer, inflammation, and autoimmune disorders.

By measuring Galectin-1 levels, researchers can gain valuable insights into disease pathology and potentially identify new therapeutic targets.Overall, the Rat Galectin-1 ELISA Kit is a valuable tool for studying the function of Galectin-1 in rat models and advancing our understanding of its role in health and disease.

Product Name:Rat Galectin 1 (GAL1) ELISA Kit
Product Code:RTDL00406
Size:96 Assays
Target:GAL1
Synonyms:LGALS1, GBP, HLBP14, Galaptin, Lectin,Galactoside-Binding Soluble 1, 14 kDa laminin-binding protein, Lactose-binding lectin 1, Putative MAPK-activating protein PM12, S-Lac lectin
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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