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Rat G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit

SKU:
AEES00413
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Rat G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit

The Rat G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit is a specialized assay designed for the quantitative analysis of G6PD levels in various biological samples from rats. Glucose 6 Phosphate Dehydrogenase serves a crucial role in cellular metabolism by catalyzing the conversion of glucose-6-phosphate to 6-phosphogluconolactone, generating NADPH and promoting the maintenance of cellular redox balance and biosynthetic processes. Any dysregulation in G6PD expression or activity can impact redox signaling, oxidative stress responses, and metabolic pathways, potentially influencing cellular health and disease states. This ELISA kit provides a precise and reliable method for quantifying G6PD levels, offering valuable insights into the enzyme's function in metabolic processes, antioxidant defenses, and cellular physiology.

By accurately measuring G6PD concentrations, researchers can gain a better understanding of its involvement in redox homeostasis, energy metabolism, and the pathophysiology of various diseases related to oxidative stress and metabolic disorders. The Rat G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, enabling reproducible and accurate results. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for researchers investigating G6PD's role in cellular metabolism, redox balance, and disease pathogenesis.

Product Name:Rat G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit
Product Code:AEES00413
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat G6PD in samples. No significant cross-reactivity or interference between Rat G6PD and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat G6PD concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat G6PD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat G6PD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat G6PD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat G6PD. You can calculate the concentration of Rat G6PD in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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