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Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit

SKU:
AEES00418
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit

The Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit is a specialized assay designed to quantitatively measure G-CSF levels in various biological samples from rats. G-CSF is a crucial cytokine that promotes the production, differentiation, and activation of granulocytes, particularly neutrophils, in response to infection or injury. This cytokine plays a key role in regulating leukocyte development and mobilization, contributing to the body's immune defense mechanisms. This ELISA kit provides researchers with a precise and reliable tool to assess G-CSF concentrations, offering valuable insights into the regulation of granulopoiesis and inflammatory responses.

By accurately quantifying G-CSF levels, scientists can further explore its functions in hematopoiesis, inflammation, and immune-mediated conditions. The Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly operation, making it an ideal choice for research purposes in immunology, hematology, and related fields studying G-CSF biology in rat models.

Product Name:Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit
Product Code:AEES00418
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat G-CSF in samples. No significant cross-reactivity or interference between Rat G-CSF and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat G-CSF concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat G-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat G-CSF. You can calculate the concentration of Rat G-CSF in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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