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Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

SKU:
RTDL00374
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P61150
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
FGF12B,FHF1,Fibroblast Growth Factor Homologous Factor 1,Myocyte-Activating Factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

The Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit is a reliable and accurate tool for detecting and measuring levels of FGF12 in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.FGF12 is a key growth factor that plays a critical role in various cellular processes, including cell growth, differentiation, and survival.

Its dysregulation has been implicated in a variety of diseases, making it a valuable biomarker for studying conditions such as cancer, cardiovascular diseases, and neurological disorders. By accurately measuring FGF12 levels, researchers can gain valuable insights into disease processes and potential therapeutic targets.

Product Name:Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit
Product Code:RTDL00374
Size:96 Assays
Target:FGF12
Synonyms:FGF12B, FHF1, Fibroblast Growth Factor Homologous Factor 1, Myocyte-Activating Factor
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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