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Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

SKU:
RTDL00373
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R5L8
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
FHF3,Fibroblast Growth Factor Homologous Factor 3
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

The Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit is specifically designed for the accurate and sensitive detection of FGF11 levels in rat serum, plasma, and cell culture supernatants. This high-quality kit offers precise and reliable results, making it an essential tool for researchers in various fields.FGF11 is a vital growth factor that plays a crucial role in cell growth, development, and proliferation. It is involved in various cellular processes, making it a key player in research areas such as cancer, cardiovascular diseases, and neurodegenerative disorders.

By measuring FGF11 levels accurately, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop new therapeutic strategies.Overall, the Rat FGF11 ELISA Kit from AssayGenie provides researchers with a powerful tool for studying the role of FGF11 in health and disease, offering high sensitivity and specificity to ensure accurate and reproducible results.

Product Name:Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit
Product Code:RTDL00373
Size:96 Assays
Target:FGF11
Synonyms:FHF3, Fibroblast Growth Factor Homologous Factor 3
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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