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Rat FGF10 (Fibroblast Growth Factor 10) ELISA Kit

SKU:
AEES00398
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat FGF10 (Fibroblast Growth Factor 10) ELISA Kit

The Rat FGF10 (Fibroblast Growth Factor 10) ELISA Kit is a precise and reliable assay designed for the quantitative detection of Fibroblast Growth Factor 10 levels in rat samples. Fibroblast Growth Factor 10 is a key member of the FGF family, which plays crucial roles in cellular processes like cell proliferation, differentiation, and tissue repair. FGF10 specifically functions as a mitogenic factor involved in embryonic development, tissue homeostasis, and wound healing. Accurate measurement of FGF10 levels using this ELISA kit is essential for investigating the regulatory role of FGF10 in various physiological processes.

FGF10 dysregulation has been implicated in developmental disorders, cancer progression, and tissue repair mechanisms, making it a valuable target for research in diverse fields of biology and medicine. The Rat FGF10 ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, providing researchers with precise and reproducible results. Manufactured under strict quality control conditions, this kit delivers reliable performance, making it an excellent choice for studying the role of FGF10 in development, disease states, and therapeutic interventions.

Product Name:Rat FGF10 (Fibroblast Growth Factor 10) ELISA Kit
Product Code:AEES00398
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat FGF10 in samples. No significant cross-reactivity or interference between Rat FGF10 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat FGF10 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FGF10. You can calculate the concentration of Rat FGF10 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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