null

Rat Ferrochelatase (FECH) ELISA Kit

SKU:
RTDL00367
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZBM3
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
EPP,FCE,Protoheme Ferrolyase,Protoporphyria,Heme synthase,Protoheme ferro-lyase
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Ferrochelatase (FECH) ELISA Kit

The Rat Ferrochelatase (FECH) ELISA Kit is a powerful tool for the precise measurement of ferrochelatase levels in rat samples. This kit is designed with high sensitivity and specificity to guarantee accurate and consistent results, making it perfect for various research purposes.Ferrochelatase is an essential enzyme involved in heme biosynthesis, playing a critical role in the synthesis of hemoglobin and other heme-containing proteins.

Dysregulation of ferrochelatase has been linked to various disorders, including anemia and porphyrias, making it a valuable target for research and potential therapeutic interventions.The Rat Ferrochelatase (FECH) ELISA Kit is a reliable tool for studying the role of ferrochelatase in health and disease, offering researchers a precise and convenient method for measuring enzyme levels in rat samples.

Product Name:Rat Ferrochelatase (FECH) ELISA Kit
Product Code:RTDL00367
Size:96 Assays
Target:FECH
Synonyms:EPP, FCE, Protoheme Ferrolyase, Protoporphyria, Heme synthase, Protoheme ferro-lyase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products