Rat Ferritin, Heavy Polypeptide (FTH) ELISA Kit ELISA Kit
SKU:
RTDL00397
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19132
Sensitivity:
1.37ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
FTH1,FTHL6,PIG15,PLIF,Ferritin Heavy Chain,Apoferritin,Placenta Immunoregulatory Factor,Proliferation-Inducing Protein 15,Cell proliferation-inducing gene 15 protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Rat Ferritin, Heavy Polypeptide (FTH) ELISA Kit ELISA Kit
The Rat Ferritin Heavy Polypeptide (FTH) ELISA Kit is specifically designed for the quantitative detection of rat ferritin heavy polypeptide in biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Ferritin is a key protein involved in iron storage and regulation, playing a critical role in maintaining iron homeostasis within the body. Abnormal levels of ferritin have been associated with various health conditions such as iron overload disorders, anemia, and neurodegenerative diseases.
Therefore, the measurement of ferritin levels can provide valuable insights into these diseases and guide potential therapeutic interventions.With its high performance and ease of use, the Rat FTH ELISA Kit is an indispensable tool for researchers studying iron metabolism, oxidative stress, and related disorders in rat models. Trust in this kit to deliver accurate and reproducible results, advancing your research in the field of ferritin biology.
Product Name:
Rat Ferritin, Heavy Polypeptide (FTH) ELISA Kit ELISA Kit
Product Code:
RTDL00397
Size:
96 Assays
Target:
FTH
Synonyms:
FTH1, FTHL6, PIG15, PLIF, Ferritin Heavy Chain, Apoferritin, Placenta Immunoregulatory Factor, Proliferation-Inducing Protein 15, Cell proliferation-inducing gene 15 protein
Detection Method:
Sandwich
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
