The Rat Ferritin (FE) ELISA Kit is a specialized assay designed for the quantitative measurement and detection of ferritin levels in various biological samples obtained from rats.
Ferritin is an essential intracellular protein that plays a crucial role in iron storage and release, which is vital for maintaining iron homeostasis, and acts as a biomarker for iron status and metabolism. This ELISA kit enables researchers to accurately measure ferritin concentrations, providing valuable insights into iron metabolism, storage, and utilization within the body. By offering exceptional sensitivity and specificity, this kit allows for precise and reliable quantification of ferritin levels, which is essential for understanding iron-related processes, such as iron deficiency, overload, and associated disorders. Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust and reproducible results, ensuring accuracy and consistency in ferritin level measurements. With its user-friendly protocols and high performance, this kit is an excellent choice for research studies investigating iron metabolism, iron-related disorders, and the role of ferritin in maintaining overall iron homeostasis in rat models.
Product Name:
Rat FE (Ferritin) ELISA Kit
SKU:
AEES00532
Target:
Rat FE (Ferritin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FE. You can calculate the concentration of Rat FE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
86-96
94-104
Average (%)
101
91
98
1:4
Range (%)
86-99
90-103
89-100
Average (%)
91
96
96
1:8
Range (%)
85-93
84-97
98-111
Average (%)
90
90
104
1:16
Range (%)
95-104
89-97
93-103
Average (%)
101
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
100-109
103
Cell culture media (n=5)
82-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.7
109.38
474.75
40.17
105.34
423.99
Standard deviation
2.58
5.01
22.31
2.24
4.75
17.6
C V (%)
5.65
4.58
4.7
5.58
4.51
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FE in samples. No significant cross-reactivity or interference between Rat FE and analogues was observed.
