Rat FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
The Rat FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit is a specialized assay designed for the precise and sensitive quantification of FABP1 levels in various biological samples obtained from rat liver tissues. FABP1, also known as liver FABP, is a protein predominantly expressed in the liver with essential functions in lipid metabolism and fatty acid transport. This ELISA kit provides researchers with a reliable tool to measure FABP1 concentrations, offering valuable insights into the liver's metabolic processes, lipid homeostasis, and liver-specific metabolic disorders.
Accurate quantification of FABP1 is crucial for studying hepatic lipid transport, fatty acid metabolism, and its implications in liver diseases such as non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Manufactured under rigorous quality control standards, the Rat FABP1 ELISA Kit ensures robust performance, precise results, and reproducibility. Its exceptional sensitivity and specificity make it an ideal choice for researchers investigating liver-related metabolic pathways and disorders in rat models.
Product Name:
Rat FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
SKU:
AEES00547
Target:
Rat FABP1 (Fatty Acid Binding Protein 1, Liver)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FABP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FABP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FABP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FABP1. You can calculate the concentration of Rat FABP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
84-95
91-102
Average (%)
97
91
95
1:4
Range (%)
87-101
98-112
92-107
Average (%)
95
104
100
1:8
Range (%)
86-101
99-109
90-100
Average (%)
93
102
97
1:16
Range (%)
95-109
83-94
98-105
Average (%)
103
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
103
EDTA plasma (n=5)
84-97
92
Cell culture media (n=5)
84-93
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.06
5.63
22.21
2.04
5.36
22.54
Standard deviation
0.1
0.26
1.12
0.12
0.26
1.17
C V (%)
4.85
4.62
5.04
5.88
4.85
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FABP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FABP1 in samples. No significant cross-reactivity or interference between Rat FABP1 and analogues was observed.
