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Rat F9 (Coagulation Factor Ⅸ) ELISA Kit

SKU:
AEES00358
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat F9 (Coagulation Factor Ⅸ) ELISA Kit

The Rat F9 (Coagulation Factor IX) ELISA Kit is a cutting-edge assay meticulously developed for the precise quantitative evaluation of Coagulation Factor IX levels in diverse rat biological samples.

Coagulation Factor IX, also known as Christmas Factor, is a key component of the coagulation cascade, crucial for proper blood clot formation and coagulation regulation. This ELISA kit empowers researchers to accurately quantify the concentration of Coagulation Factor IX, offering valuable insights into its role in coagulation disorders, hemophilia B, and various bleeding disorders. Accurate measurement of Coagulation Factor IX is fundamental for unraveling its significance in coagulation pathways, understanding hemostasis, and identifying potential therapeutic interventions for bleeding disorders. With the Rat F9 ELISA Kit, researchers can explore the intricate mechanisms of blood coagulation, advancing the knowledge in coagulation-related research and clinical diagnostics. Assay Genie's Rat F9 ELISA Kit boasts exceptional sensitivity and specificity, ensuring dependable and reproducible outcomes. Manufactured under stringent quality standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for researchers investigating Coagulation Factor IX's functions in both health and disease states.

Product Name:Rat F9 (Coagulation Factor Ⅸ) ELISA Kit
Product Code:AEES00358
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat F9 in samples. No significant cross-reactivity or interference between Rat F9 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat F9 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat F9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat F9. You can calculate the concentration of Rat F9 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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