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Rat F10 (Coagulation Factor Ⅹ) ELISA Kit

SKU:
AEES00357
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.38 ng/mL
Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Rat F10 (Coagulation Factor Ⅹ) ELISA Kit

The Rat F10 (Coagulation Factor X) ELISA Kit is an advanced assay designed for the precise quantitative assessment of Coagulation Factor X levels in a variety of rat biological samples. Coagulation Factor X, a pivotal player in the blood coagulation cascade, contributes to the formation of blood clots and is crucial for maintaining hemostasis. This ELISA kit enables researchers to accurately measure the concentration of Coagulation Factor X, providing valuable insights into its role in coagulation disorders, thrombosis, and various cardiovascular conditions. Accurate quantification of Coagulation Factor X is essential for comprehending its involvement in the coagulation pathway, fibrin clot formation, and clotting abnormalities.

By utilizing this ELISA kit, researchers can explore the intricate mechanisms underlying blood coagulation, which can inform clinical diagnostics and the development of targeted treatment strategies for coagulation-related diseases and conditions. The Rat F10 ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, ensuring trustworthy and reproducible results. With stringent quality control measures in place, this kit has been manufactured to deliver reliable performance and user-friendly operation, making it an excellent research tool for investigating the functions and dysregulations of Coagulation Factor X in both normal and pathological states.

Product Name:Rat F10 (Coagulation Factor Ⅹ) ELISA Kit
Product Code:AEES00357
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.63-40 ng/mL
Sensitivity:0.38 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat F10 in samples. No significant cross-reactivity or interference between Rat F10 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat F10 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat F10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat F10. You can calculate the concentration of Rat F10 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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